Table 2.
Taxonomic identity of putative N2-fixing bacteria isolates obtained by sequencing of 16S rRNA genes.
| Isolate | Taxonomy groupa | Closest relatives or cloned sequences (accession no.)b | Identity | Accession no. |
|---|---|---|---|---|
| RHIZOSPHERE | ||||
| 62CR | Actinobacteria; Micrococcales; Microbacteriaceae; Microbacterium | Microbacterium proteolyticum from Halimione portulacoides roots (NR_132869) | 99% | MG835569 |
| 72CR | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus megaterium strain p102_H06 from maíze rhizosphere (JQ832067) | 99% | MG835570 |
| 102BR | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Nitrogen-fixing Bacillus megaterium strain ADU08 from date palm soil (KX694270) | 99% | MG835571 |
| 112BR | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus megaterium strain ARD47 from acid soil (KX023249) | 100% | MG835572 |
| 154AR | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus sp. strain SZ177 from straw decomposition (KU986708) | 99% | MG835573 |
| 173CR | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus megaterium strain PSC1 from sugarcane rhizosphere (KU196781) | 99% | MG835574 |
| 184AR | Actinobacteria; Micrococcales; Microbacteriaceae; Microbacterium | Diazotrophic Microbacterium sp. S2SP302 from sugarcane rhizosphere (KT183549) | 100% | MG835575 |
| 184AR-1 | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus megaterium strain yangyueK8 from Juglans regia rhizosphere (KU977121) | 100% | MG835576 |
| 214AR | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus megaterium strain yangyueN10 from Juglans regia rhizosphere (KU977110) | 100% | MG835577 |
| 214AR-1 | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus megaterium strain yangyueN10 from Juglans regia rhizosphere (KU977110) | 100% | MG835578 |
| 222BR | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus sp. MR35 from rice rhizosphere (LT629146) | 99% | MG835579 |
| 623EA | Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Chitinophaga | Chitinophaga arvensicola strain MRP-16 from Dioscorea batatas rhizoplane (AB908086) | 99% | MG835588 |
| 243AR | Actinobacteria; Micrococcales; Micrococcaceae; Arthrobacter | Arthrobacter sp. strain JQ-1 from phtalate contaminated soil (KX055564) | 100% | MG835580 |
| 322CR | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus megaterium strain ARD47 from acid soil (KX023249) | 100% | MG835583 |
| 342CR | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus huizhouensis strain WJB150 from rice rhizosphere 1(KU877672) | 100% | MG835584 |
| 354AR | Actinobacteria; Micrococcales; Microbacteriaceae; Microbacterium | Microbacterium sp. SIB_Cu_R3 from Betula pendula L. rhizosphere (KX036571) | 100% | MG835585 |
| 354AR-1 | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus megaterium strain Hd from arsenic contaminated soil (KY098770) | 100% | MG835586 |
| 372EC | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Nitrogen-fixing Bacillus megaterium strain ADU08 from date palm soil (KX694270) | 99% | MG835587 |
| 503CR | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus aryabhattai strain He from arsenic contanimated soil (KY098771) | 100% | MG835581 |
| 503CR-1 | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus amyloliquefaciens strain Y14 from peanut rhizosphere (CP017953) | 99% | MG835582 |
| ROOT ENDOSPHERE | ||||
| 223EC | Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium | Mycobacterium sp. Site1-11a from manure-fertilized grassland (JF304596) | 99% | MG835593 |
| 382EC | Actinobacteria; Micrococcales; Microbacteriaceae; Microbacterium | Microbacterium sp. SIB_Cu_R3 from Betula pendula L. rhizosphere (KX036571) | 100% | MG835590 |
| 424EC | Actinobacteria; Micrococcales; Bogoriellaceae; Georgenia | Georgenia soli strain MC-14-2 from Populus euphratica endosphere (KF848489) | 99% | MG835591 |
| 444EC | Firmicutes; Bacilli; Bacillales; Bacillaceae; Psychrobacillus | Psychrobacillus psychrodurans strain EC1 from corn roots (KP334982) | 99% | MG835592 |
| 491EC | Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium | Mycobacterium sp. strain OTB74 from Sandy soil (KN022865) | 99% | MG835594 |
| 513EC | Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseomonas | Roseomonas sp. A2 from tomato rhizosphere (KP789481) | 99% | MG835595 |
| 523EC | Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseomonas | Roseomonas sp. Esch5-313 from poplar endosphere (AM489616) | 99% | MG835596 |
| 543EC | Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseomonas | Roseomonas sp. strain THG-N2.22 from soil (KX456186) | 98% | MG835598 |
| 564EB | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus amyloliquefaciens strain Y14 from peanut rhizosphere (CP017953) | 100% | MG835599 |
| 584EA-1 | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus aryabhattai strain He from arsenic contanimated soil (KY098771) | 100% | MG835600 |
| 592BR | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus aryabhattai strain He from arsenic contanimated soil (KY098771) | 100% | MG835601 |
| 643EA | Bacteroidetes; Chitinophagia; Chitinophagales; Chitinophagaceae; Chitinophaga | Chitinophaga sp. W-9 from tomato rhizosphere (KX082640) | 99% | MG835602 |
| 693EB | Proteobacteria; Alphaproteobacteria; Rhizobiales; Bradyrhizobiaceae; Bosea | Bosea sp. strain SR 5-12 from Artemisa prínceps endorhiza (KM253172) | 100% | MG835603 |
| 703EB | Proteobacteria; Alphaproteobacteria; Rhizobiales; Bradyrhizobiaceae; Bosea | Bosea sp. strain J1 from soil (KP125320) | 100% | MG835604 |
| 274EB | Actinobacteria; Micrococcales; Microbacteriaceae; Leifsonia | Leifsonia sp. URHA0017 from grassland rhizosphere (LN876290) | 99% | MG835589 |
| 223EC | Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium | Mycobacterium sp. Site1-11a from manure-fertilized grassland (JF304596) | 99% | MG835593 |
| 714EA | Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus | Bacillus megaterium strain Hd (KY098770) | 100% | MG835605 |
| 734EA | Actinobacteria; Micrococcales; Bogoriellaceae; Georgenia | Georgenia soli strain CC-NMPT-T3 from iron ore-contaminated soil (NR_116959) | 98% | MG835606 |
a
The phylogenetic assignment is based on sequence analysis by BLASTn of GenBank database from NCBI (http://www.ncbi.nlm.nih.gov). It is given the phylum as well as the lowest predictable phylogenetic rank.
b
Based on partial sequencing of 16S rRNA gene and comparison with those present in GenBank by using BLASTn algorithm.