Figure 2.

Inflammation increased lipid accumulation to accelerate tubulointerstitial injuries. Eight-week-old diabetic db/db mice were randomly assigned and received subcutaneous injections with either 0.5 mL distilled water (db/db, n=10) or 0.5 mL 10% casein (db/db+casein, n=10) every other day for 8 weeks. HK-2 cells were made quiescent using serum-free medium for 24 h, and treated with 30 mmol/L glucose (HG) or 30 mmol/L glucose plus 5 ng/mL IL-1β. TG, triglyceride; TC, total cholesterol; LDL, low-density lipoprotein; HDL, high-density lipoprotein (A). Filipin staining was used to examine lipid accumulation both in vivo and in vitro (B and D, original magnification ×200). The concentration of cholesterol ester was measured both in vivo (C, n=5) and in vitro (E). The results represent the mean±SD. ^*P<0.05 vs db/db or HG group.