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. 2018 Nov 9;11:405. doi: 10.3389/fnmol.2018.00405

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Copyright © 2018 Ha, Leal-Ortiz, Lalwani, Kiyonaka, Hamachi, Mysore, Montgomery, Garner, Huguenard and Kim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Zinc treatment recruits surface GluA2 and disperses surface GluA1. (A) Straightened dendrites from young hippocampal neurons (DIV 14) treated with control (left) or 10 μM ZnCl₂ (right) conditions. Neurons were live surface labeled for GluA1 (top) or GluA2 (bottom). Scale bar: 4 μm. (B–D) Summary graphs showing quantification of GluA1 and GluA2 puncta intensity (B), density (C) and volume (D) [mean ± SEM; Mann-Whitney test for GluA1, N = 19 (control), 22 (ZnCl₂) dendrites from 10 to 14 neurons from two culture preps; for GluA2, N = 24 dendrites from 10 to 14 neurons from two culture preps; n.s. p ≥ 0.05, **p < 0.01, ***p < 0.001].