Figure 8.

Zinc treatment alters the colocalization between AMPAR subunits and Shank. (A) Straightened dendrites from young hippocampal neurons (DIV 14) treated with control (left) or 10 μM ZnCl₂ (right). Neurons were live surface labeled for GluA1 (green; top) or GluA2 (green; bottom) before being fixed and co-immunostained for both Shank2 (red) and Shank3 (not shown). Yellow indicates colocalization. Scale bar: 4 μm. (B–E) Three-dimensional colocalization analysis of pairwise puncta overlap using IMFLAN3D as measured by the fraction of overlap (B,C), intensity (D) and volume (E) for baseline and ZnCl₂ conditions (mean ± SEM) [Mann-Whitney test, for GluA1 + Shank3 or Shank2, N = 19 (control) and 22 (ZnCl₂) dendrites from 10 to 14 neurons from two culture preps; for GluA2 + Shank3 or Shank2, N = 24 dendrites from 10 to 14 neurons from two culture preps; for Shank2 + Shank3, N = 43 (control) and 46 (ZnCl₂) dendrites from 20 to 25 neurons from four culture preps; n.s. p ≥ 0.05, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001].