Figure 2.

Overexpression of HDAC6 suppresses the proliferation of HCC cells and the activity of canonical Wnt/β-catenin signaling. (A) The proliferation ability of HCC cells (Hep3B and Huh-7) was analyzed using a Cell Counting Kit-8 assay. HCC cells were transfected with 4 µg P3-HDAC6 plasmid (P3-HDAC6) or an empty plasmid (Mock). Error bars represent the standard deviations of three independent experiments. *P<0.01 and ^#P<0.05 vs. mock. (B) The protein levels of HDAC6 and the canonical Wnt/β-catenin signaling cascade proteins (β-catenin, cyclin D1 and Myc) and cleaved caspase 3 were detected using western blot analysis for three HCC cell lines. HCC, hepatocellular carcinoma; HDAC6, histone deacetylase 6.