Figure 5.

Nested PCR for plasma membrane calcium ATPase 2 using specific RT-qPCR oligos revealed single bands of the expected size, confirming the specificity of the PCR products: 2w, 152 bp; 2w, 104 bp; 2×, 80 bp; 2z, 73 bp. RT-PCR was performed using total RNA isolated from tissue adjacent to the tumor (N). For electrophoresis, PCR products were run on 3% agarose gel. The sizes of a number of DNA molecular weight markers are depicted. bp, base pairs; +, with cDNA; -, without cDNA; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.