Table II.
Primers for PMCA isoforms expression analysis using quantitative polymerase chain reaction analysis and for normal reverse transcription-polymerase chain reaction (RT-PCR) (5AF-5AR; 8BF-8BR).
| Splicing isoform site | Strand | Sequence (5′→3′) | Amplicon size (bp) | Isoform |
|---|---|---|---|---|
| Oligo name for PMCA-2 Site A | ||||
| 1AF | Forward | GTGTGAAGAAGGGGGATGGC | 104 | 2w |
| 1AR | Reverse | CTGCATTTTACCATTGACTAGGCTGG | ||
| 2AF | Forward | TCAGACTGGCATCATCTTTACCCTCC | 80 | 2× |
| 2AR | Reverse | CCTGCATTTTACCTTTTTTGTCTTTCTTCTC | ||
| 3AF | Forward | GTGTGAAGAAGGGGGATGGC | 109 | 2y |
| 3AR | Reverse | CCGTCCTGTTGTTTGGCATTGAC | ||
| 4AF | Forward | GACAAAAAAGCCAAACAACAGGACGG | 73 | 2z |
| 4AR | Reverse | CTCGGCACTCTTGAGGGGCTG | ||
| 5AF | Forward | TGACTGCTGTGGGTGTG | 363 | 2w |
| 5AR | Reverse | CAGCTTGGTGAGCTTGC | 270 | 2× |
| 320 | 2y | |||
| 228 | 2z | |||
| Oligo name for PMCA-2 Site C | ||||
| 6BF | Forward | GCCCTAGTCGCGTGTCGTTGTCC | 77 | 2a |
| 6BR | Reverse | CTAGCCCTGCCCGGCTGACG | ||
| 7BF | Forward | GGCAGGCAGGCTCACACAGAAG | 52 | 2b |
| 7BR | Reverse | CACGACGCGGATCTGTGTCTGG | ||
| 8BF | Forward | CACCATCCCTACCAGCAGGCT | 584 | 2a |
| 357 | 2b | |||
| 529 | 2c | |||
| 8BR | Reverse | GCTCGAGTTCTGCTTGAGCGC | ||
| Housekeeping oligo name | ||||
| 9HF | Forward | TACTTGGATAACTGTGGTAATTCTAGAG | 110 | 18S |
| 9HR | Reverse | AGGGGCTGACCGGGTTGG | rRNA |
PMCA2, plasma membrane calcium ATPase 2; bp, base pair.