Table II.
Analytical imprecision of the HPV16 (E2 and E6 genes) and HPV18 (E2 and E6 genes) measurement using multiplex quantitative polymerase chain reaction.
| HPV16 E2 gene | HPV16 E6 gene | |||
|---|---|---|---|---|
| Precision | Mean ± SD (CV%) Cq | Mean ± SD (CV%) Copy number | Mean ± SD (CV%) Cq | Mean ± SD (CV%) Copy number |
| Within-run (n=12)a | ||||
| Low levelc | 32.41±0.18 (0.54) | 961±127.1 (13.23) | 33.67±0.17 (0.52) | 1,084±149.1 (13.74) |
| High leveld | 23.11±0.08 (0.35) | 952,490±59,040.35 (6.19) | 24.59±0.10 (0.39) | 1,097,119±81,784.1 (7.45) |
| Between-run (n=15)b | ||||
| Low levelc | 32.61±0.20 (0.60) | 973±141.36 (14.53) | 34.26±0.19 (0.54) | 929±137.45 (14.79) |
| High leveld | 23.37±0.14 (0.59) | 911,140±91,626.87 (10.06) | 25.04±0.14 (0.55) | 1,040,597±112,028.74 (10.77) |
| HPV18 E2 gene | HPV18 E6 gene | |||
| Precision | Mean ± SD (CV) Cq | Mean ± SD (CV) Copy number | Mean ± SD (CV) Cq | Mean ± SD (CV) Copy number |
| Within-run (n=12)a | ||||
| Low levelc | 33.47±0.17 (0.52) | 1,159±160.1 (13.81) | 34.83±0.18 (0.52) | 1,067±144.35 (13.52) |
| High leveld | 24.61±0.07 (0.29) | 1,123,790±60,126.32 (5.4) | 25.73±0.11 (0.44) | 1,023,242±86,472.54 (8.45) |
| Between-run (n=15)b | ||||
| Low levelc | 34.03±0.19 (0.57) | 996±147.1 (14.76) | 34.77±0.19 (0.53) | 958±134.18 (14.00) |
| High leveld | 25.00±0.11 (0.43) | 992,011±81,890.14 (8.25) | 25.42±0.13 (0.51) | 1,054,498±101,053.01 (9.58) |
a
12 replicates per experiment
b
Triplicate in 5 independent experiments
c
103 copies of HPV16 and HPV18 DNA
d
106 copies of HPV16 and HPV18 DNA. HPV, human papillomavirus; Cq, quantification cycle.