Table IV.

Competitive effect of unequal copies between HPV16 and HPV18 on the measurement of HPV18 E2 and E6 genes using multiplex quantitative polymerase chain reaction.

HPVE2 gene				HPVE6 gene
Unequal mixture HPV18 and HPV16		Single HPV18		Unequal mixture HPV18 and HPV16		Single HPV18
Copy ratio	Measured copies	Copy of	Measured copies of	Copy ratio	Measured copies	Copy of	Measured copies of
(HPV18:HPV16)	of HPV18E2 (Test)a	HPV18	HPV18E2 (Control)a	(HPV18:HPV16)	of HPV18E6 (Test)a	HPV18	HPV18E6 (Control)a
106:103	1,135,661	106	1,075,620	106:103	1,015,464	106	1,107,241
106:104	1,097,976	106	1,181,450	106:104	1,107,170	106	1,125,579
106:105	877,385	106	1,135,265	106:105	911,742	106	893,768
106:106	1,092,086	106	993,922	106:106	965,678	106	1,040,114
105:103	104,611	105	96,998	105:103	90,546	105	94,778
105:104	101,164	105	113,785	105:104	97,952	105	113,933
105:105	105,464	105	101,469	105:105	110,175	105	87,996
105:106	102,760	105	113,715	105:106	87,492	105	107,587
104:103	9,129	104	8,789	104:103	10,201	104	9,746
104:104	9,550	104	9,443	104:104	9,732	104	10,395
104:105	8,639	104	9,988	104:105	10,403	104	8,665
104:106	10,700	104	9,812	104:106	8,910	104	10,550
103:103	912	103	1,120	103:103	937	103	1,056
103:104	936	103	1,078	103:104	910	103	938
103:105	849	103	831	103:105	901	103	833
103:106	896	103	1,168	103:106	869	103	1,063

^aNon-significant difference between test and control for HPV18E2 (P=0.526; paired t-test) and HPV18E6 (P=0.146; paired t-test). HPV, human papillomavirus.