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. 2018 Dec;24(12):2241–2250. doi: 10.3201/eid2412.180937

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Serologic testing for HEV infection at Queen Mary Hospital, Hong Kong. A) Sodium dodecyl sulfate polyacrylamide gel electrophoresis gel showing purified HEV-A and HEV-C 239-aa recombinant proteins used in Western blot and ELISA. Lane 1, molecular weight marker; lane 2, HEV-A protein; lane 3, HEV-C protein. B–C) IgM and IgG Western blot using HEV-A protein (B) and HEV-C protein (C). Lane 1, patient serum (posttransplant day 100); lanes 2–5, individual platelet donor serum; lane 6, organ donor serum; lane 7, murine polyclonal serum against HEV-C; lane 8, specific monoclonal antibody against HEV-A; lane 9, cross-reactive monoclonal antibody against HEV-A and HEV-C. D, E) HEV-A and HEV-C ELISA IgG titers of patient pretransplant (D) and posttransplant serum (E) using an OD of 0.3 as assay cutoff as described in the Technical Appendix. HEV, hepatitis E virus; HEV-A, Orthohepevirus A; HEV-C, Orthohepevirus C; OD, optical density.