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. 2018 Dec;24(12):2241–2250. doi: 10.3201/eid2412.180937

Figure 5.

Figure 5

Isolation of HEV-C from 56-year-old male patient’s feces in cell culture, Queen Mary Hospital, Hong Kong. A) HEV-C RNA loads in culture S and day-7 CL of A549, Huh-7, and Caco-2 cell lines after inoculation by patient’s filtered fecal suspension. Mean of 3 replicates; error bars indicate SEM. B) Uninfected A549 cell monolayer stained with anti–HEV-C polyclonal antiserum. C) Infected A549 cell monolayer stained with anti–HEV-C polyclonal antiserum. Original magnification ×400. CL, cell lysate; HEV, hepatitis E virus; S, supernatant.