Table 10.
Absolute values of peritoneal leucocytes, lymphocytes, macrophages, and neutrophils of European seabass fed dietary treatments at 4, 24, and 48 h after infection.
| Parameters | Dietary treatments | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| CTRL | MET 0.5 | MET 1 | |||||||
| 4 h | 24 h | 48 h | 4 h | 24 h | 48 h | 4 h | 24 h | 48 h | |
| Leucocytes (× 104 μl−1) | 8.70 ± 3.59 | 5.75 ± 1.82 | 10.90 ± 2.57b | 6.68 ± 2.03 | 14.40 ± 6.28 | 10.88 ± 1.51b | 12.62 ± 5.99* | 10.57 ± 4.03* | 22.00 ± 4.57a |
| Lymphocytes (× 104 μl−1) | 2.37 ± 1.04 | 1.73 ± 0.88 | 1.45 ± 0.42 | 1.80 ± 1.20 | 1.63 ± 0.58 | 2.55 ± 1.07 | 2.28 ± 1.31* | 4.85 ± 1.75 | 5.07 ± 2.15 |
| Macrophages (× 104 μl−1) | 6.58 ± 2.08 | 5.51 ± 2.80 | 5.25 ± 1.61 | 4.26 ± 3.43 | 3.00 ± 0.86 | 4.06 ± 1.76 | 4.12 ± 1.07 | 8.83 ± 1.52 | 8.16 ± 2.71 |
| Neutrophils (× 104 μl−1) | 1.77 ± 0.73 | 0.88 ± 0.37b | 2.02 ± 0.53b | 1.53 ± 0.44 | 3.37 ± 1.37a | 1.69 ± 0.19b | 3.52 ± 1.75 | 3.10 ± 1.39a | 4.35 ± 1.64a |
| Two-way ANOVA | |||||||||
| Parameters | Time | Diet | |||||||
| Time | Diet | Time × Diet | 4 h | 24 h | 48 h | CTRL | MET 0.5 | MET 1 | |
| Leucocytes | <0.001 | <0.001 | 0.003 | B | B | A | B | B | A |
| Lymphocytes | 0.028 | ns | 0.034 | B | A | AB | – | – | – |
| Macrophages | <0.001 | 0.005 | ns | C | B | A | B | B | A |
| Neutrophils | ns | <0.001 | 0.025 | – | – | – | B | B | A |
Values are presented as means ± SD (n = 6). P-values from two-way ANOVA (p ≤ 0.05). If interaction was significant, Tukey post-hoc test was used to identify differences in the experimental treatments. Different lowercase letters stand for significant differences among dietary treatments for the same time while different symbols stands for significant differences between times for the same diet. Different capital letters indicate differences among times regardless diets and among diets regardless time.