Table 6.
Absolute values of peripheral blood leucocytes (thrombocytes, lymphocytes, monocytes, and neutrophils) of European seabass fed dietary treatments during 2 and 4 weeks.
| Parameters | Dietary treatments | |||||
|---|---|---|---|---|---|---|
| CTRL | MET 0.5 | MET 1 | ||||
| 2 weeks | 4 weeks | 2 weeks | 4 weeks | 2 weeks | 4 weeks | |
| Thrombocytes (× 104 μl−1) | 4.16 ± 1.05 | 2.93 ± 0.50 | 4.99 ± 2.19 | 2.71 ± 0.88 | 4.68 ± 1.03 | 2.96 ± 0.81 |
| Lymphocytes (× 104 μl−1) | 3.63 ± 0.89 | 1.58 ± 0.40 | 4.88 ± 2.20 | 2.07 ± 0.52 | 4.40 ± 1.21 | 2.52 ± 0.91 |
| Monocytes (× 104 μl−1) | 0.23 ± 0.08 | 0.12 ± 0.05 | 0.23 ± 0.14 | 0.15 ± 0.09 | 0.25 ± 0.10 | 0.20 ± 0.13 |
| Neutrophils (× 104 μl−1) | 0.06 ± 0.07 | 0.03 ± 0.02 | 0.02 ± 0.02 | 0.08 ± 0.11 | 0.12 ± 0.04 | 0.17 ± 0.12 |
| Two-way ANOVA | ||||||
| Parameters | Diet | |||||
| Time | Diet | Time × Diet | CTRL | MET 0.5 | MET 1 | |
| Thrombocytes | <0.001 | ns | ns | – | – | – |
| Lymphocytes | <0.001 | ns | ns | – | – | – |
| Monocytes | 0.018 | ns | ns | – | – | – |
| Neutrophils | ns | 0.004 | ns | B | B | A |
Values are presented as means ± SD (n = 9). P-values from two-way ANOVA (p ≤ 0.05) (n = 9). If interaction was significant, Tukey post-hoc test was used to identify differences in the experimental treatments. Different capital letters indicate differences among diets regardless time.