Table 8.
Hemoglobin, mean corpuscular hemoglobin (MCH), red blood cells (RBC), and white blood cells (WBC) in European seabass fed dietary treatments at 4 weeks (0 h), 4, 24, and 48 h after infection.
| Parameters | Dietary treatments | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTRL | MET 0.5 | MET 1 | ||||||||||
| 0 h | 4 h | 24 h | 48 h | 0 h | 4 h | 24 h | 48 h | 0 h | 4 h | 24 h | 48 h | |
| Hemoglobin (g dl) | 1.40 ± 0.68 | 1.18 ± 0.26 | 0.94 ± 0.20 | 1.46 ± 0.30 | 1.23 ± 0.40 | 1.01 ± 0.22 | 0.93 ± 0.21 | 1.16 ± 0.23 | 1.18 ± 0.49 | 1.40 ± 0.47 | 1.23 ± 0.34 | 1.27 ± 0.14 |
| MCH (pg cell−1) | 8.64 ± 2.47 | 6.81 ± 1.35 | 4.91 ± 0.96 | 6.43 ± 0.84 | 6.51 ± 1.82 | 6.44 ± 1.52 | 4.78 ± 0.63 | 5.02 ± 1.06 | 6.21 ± 2.53 | 7.92 ± 3.31 | 6.37 ± 2.47 | 6.04 ± 1.45 |
| RBC (× 106 μl−1) | 1.83 ± 0.34 | 1.76 ± 0.32 | 1.93 ± 0.38 | 2.26 ± 0.30 | 1.92 ± 0.42 | 1.57 ± 0.15 | 1.95 ± 0.33 | 2.35 ± 0.37 | 1.95 ± 0.39 | 1.83 ± 0.16 | 2.00 ± 0.32 | 2.16 ± 0.26 |
| WBC (× 104 μl−1) | 4.67 ± 0.80 | 6.52 ± 1.11 | 5.60 ± 1.16 | 8.53 ± 1.92 | 5.73 ± 0.65 | 6.28 ± 0.79 | 6.40 ± 1.06 | 10.17 ± 1.28 | 6.40 ± 1.45 | 6.52 ± 1.12 | 6.48 ± 0.64 | 8.95 ± 1.06 |
| Two-way ANOVA | ||||||||||||
| Time | Diet | |||||||||||
| Parameters | Time | Diet | Time × Diet | 0 h | 4 h | 24 h | 48 h | CTRL | MET 0.5 | MET 1 | ||
| Hemoglobin | ns | ns | Ns | – | – | – | – | – | – | – | ||
| MCH | ns | ns | ns | – | – | – | – | – | – | – | ||
| RBC | <0.001 | ns | ns | B | B | AB | A | – | – | – | ||
| WBC | <0.001 | 0.035 | ns | B | B | B | A | B | A | A | ||
Values are presented as means ± SD (n = 6). P-values from two-way ANOVA (p ≤ 0.05). If interaction was significant, Tukey post-hoc test was used to identify differences in the experimental treatments. Different capital letters indicate differences among times regardless diets and among diets regardless time.