Table 9.
Absolute values of peripheral blood leucocytes (i.e., thrombocytes, lymphocytes, monocytes, and neutrophils) of European seabass fed dietary treatments at 4 weeks (0 h), 4, 24, and 48 h after infection.
| Parameters | Dietary treatments | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTRL | MET 0.5 | MET 1 | ||||||||||
| 0 h | 4 h | 24 h | 48 h | 0 h | 4 h | 24 h | 48 h | 0 h | 4 h | 24 h | 48 h | |
| Thrombocytes (× 104 μl−1) | 2.93 ± 0.50 | 4.49 ± 0.69 | 3.69 ± 0.91 | 3.89 ± 0.40 | 2.71 ± 0.88 | 4.53 ± 0.88 | 4.15 ± 0.56 | 4.56 ± 0.45 | 2.96 ± 0.81 | 3.96 ± 0.80 | 3.79 ± 0.41 | 4.27 ± 0.48 |
| Lymphocytes (× 104 μl−1) | 1.58 ± 0.40 | 1.49 ± 0.32 | 1.23 ± 0.43 | 3.30 ± 1.39 | 2.07 ± 0.52 | 1.09 ± 0.24 | 1.24 ± 0.36 | 4.16 ± 0.58 | 2.52 ± 0.91 | 1.78 ± 0.56 | 1.56 ± 0.25 | 3.42 ± 0.70 |
| Monocytes (× 104 μl−1) | 0.12 ± 0.05 | 0.06 ± 0.03 | 0.15 ± 0.06 | 0.54 ± 0.27 | 0.15 ± 0.09 | 0.10 ± 0.04 | 0.19 ± 0.11 | 0.40 ± 0.15 | 0.20 ± 0.13 | 0.25 ± 0.09 | 0.18 ± 0.06 | 0.48 ± 0.13 |
| Neutrophils (× 104 μl−1) | 0.03 ± 0.02 | 0.47 ± 0.33 | 0.62 ± 0.15 | 0.89 ± 0.42 | 0.08 ± 0.11 | 0.74 ± 0.21 | 0.90 ± 0.22 | 1.10 ± 0.91 | 0.17 ± 0.12 | 0.67 ± 0.10 | 0.96 ± 0.32 | 0.91 ± 0.38 |
| Two-way ANOVA | ||||||||||||
| Time | Diet | |||||||||||
| Parameters | Time | Diet | Time × Diet | 0 h | 4 h | 24 h | 48 h | CTRL | MET 0.5 | MET 1 | ||
| Thrombocytes | <0.001 | ns | ns | B | A | A | A | – | – | – | ||
| Lymphocytes | <0.001 | ns | ns | B | C | C | A | – | – | – | ||
| Monocytes | <0.001 | ns | ns | B | B | B | A | – | – | – | ||
| Neutrophils | <0.001 | 0.045 | ns | C | B | AB | A | B | A | A | ||
Values are presented as means ± SD (n = 6). P-values from two-way ANOVA (p ≤ 0.05). If interaction was significant, Tukey post-hoc test was used to identify differences in the experimental treatments. Different capital letters indicate differences among times regardless diets and among diets regardless time.