FIG 6.
PTX and/or antimicrobial agents inhibited live microorganisms-induced TNF mRNA expression in whole newborn cord blood. Cord blood (n = 10) was stimulated with live microorganisms—E. coli at 104 CFU/ml (a), S. aureus at 105 CFU/ml (b), S. epidermidis at 105 CFU/ml (c), or C. albicans at 105 CFU/ml (d)—and simultaneously treated with 200 µM PTX and/or antimicrobial agents or vehicle control and then cultured for 4 h in 5% CO2 at 37°C. Relative mRNA expressions of samples stimulated with microorganisms and treated with PTX and/or antimicrobial agents are calculated from ΔΔCT values and normalized to microbial stimulation alone, which was defined as 100%. Significant differences between ΔΔCT values of treated versus untreated samples and between samples treated with single (PTX or antimicrobial) agents versus combination treatment are indicated (*, P < 0.05; **, P < 0.01; ***, P < 0.001).