FIG 1.

Workflow of competitive fitness assay. (A) The sensitized mutant library is grown competitively without antibiotics (ATB; control), or with the IC₂₅ of C109 or novobiocin. Growth with antibiotics selectively depletes certain mutants. (B) To track mutant abundance, transposon-genome junctions are enriched using Tn-Seq Circle. (C) Samples are sequenced on a HiSeq platform, and reads are then mapped to the B. cenocepacia K56-2 genome to call insertion sites. The reads from the antimicrobial-treated conditions are compared to the no-antibiotic controls to determine highly depleted mutants, which are used to call candidate antibacterial-target matches.