FIG 3.

C109 causes cell filamentation and disrupts divisome formation. (A and B) Localization of FtsZ (A and B), FtsA (A), and FtsW (A) upon 3-h treatment of exponentially growing ASKA strains with C109 (A and B) or cefotaxime (B). Arrowheads show areas with deficient nucleoid segregation. Chevrons show proper nucleoid segregation. (C) Time-lapse fluorescence microscopy of FtsZ-GFP cells, spotted onto an agarose pad with or without 4 μg/ml C109. Dashes denote cell boundaries. (D and E) Cell length (D) and proportion of cells with Z-ring (E) after 3 h of exposure to C109 (n = 200 cells).