TABLE 1.
Characterization of LZD resistance mechanisms and correlation with generation times
| Isolate | LZD MIC (mg/liter)a |
LZD resistance mechanismb |
Minimum generation time (mean ± SD) |
|||
|---|---|---|---|---|---|---|
| 23S rRNA gene (no. of mutated rrl alleles/total no. of rrl alleles) |
L3 protein (rplC gene) |
L4 protein (rplD gene) |
cfr gene | |||
| LimS | 0.75 | WT | WT | WT | — | 49.9 ± 0.7 |
| LimR1 | >256 | G2576T (5/5) | WT | WT | — | 260.7 ± 38.5c |
| LimR3 | 8 | G2576T (3/5) | WT | WT | — | 54.9 ± 1.9c |
| LimR6 | 8 | G2576T (4/5) | WT | WT | — | 158.0 ± 11.4 |
| LimR8 | 32 | G2576T (4/5) | WT | WT | — | 158.9 ± 16.4 |
| LimR9 | 24 | G2576T (4/5) | WT | V142A | — | 65.5 ± 4.2c |
| LimR12 | 16 | G2576T (4/5) | WT | WT | — | 67.8 ± 4.8c |
a
LZD resistance is defined by a MIC of >4 mg/liter by EUCAST and CLSI for S. aureus.
b
Determined with WGS data. WT, wild type; —, absent.
c
P ≤ 0.05, Student’s test in comparison with the results for the first isolate LimS.