FIG 4.

Sertraline effects on the respiratory chain of L. infantum promastigotes. Parasites were resuspended in respiration buffer at 10⁸ cells/ml in a Clark oxygen electrode. Succinate was used as the sole substrate. Oxygen consumption rates of L. infantum promastigotes were calculated from the corresponding slope and are expressed as nmol O₂/min. (A) Uncoupling activity of sertraline on digitonized parasites. (B) Respiration controls in untreated parasites. Traces are representative of one of three independent experiments. Reagent concentrations: 60 µM digitonin, 100 µM ADP, 12.6 µM oligomycin, 3.3 µM FCCP, and 50 µM SRT. Arrows represent the addition of the respective reagents.