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. 2018 Nov 13;35(4):283–289. doi: 10.4274/tjh.2018.0129

Figure 2. A) Demonstrating hypersegmented basophil (1), basophilic myelocyte (2), giant binuclear basophilic metamyelocyte (3), pyknotic eosinophil and basophil with drum-stick like nuclear sticks (4, 10), normal basophilic metamyelocyte (6), pyknotic myelocyte, metamyelocytes, binuclear basophilic metamyelocyte and basophilic myelocyte (5, 7, 11, 12, 16), agranular and hypogranular metamyelocyte (8, 9), binuclear hypogranular metamyelocyte (13), basophilic myelocyte (14), Pelger-Hüet anomaly (15) and aggregates of mast cells having mixed orange and dark purplish to black color round cytoplasmic granules (17) in accelerated phase of primary chronic basophilic leukemia with mast cell leukemia (Wright’s stain, 100x); B) Showing tryptase activity in the round, brown color of cytoplasmic granules of mast cells demonstrated by immunohistochemical staining for tryptase. (tryptase immunohistochemical staining, 100x); C) Demonstrating black granular cytoplasmic staining by peroxidase stain in myeloperoxidase-positive basophils and absence of staining in aggregates of cells representing myeloperoxidase-negative mast cells in the bone marrow (peroxidase stain, 100x).

Figure 2