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Effect of siLRG1 plasmid transfection on cell apoptosis. (A–D) Flow cytometry was applied to detect the cell apoptosis of each group after transfection. (E, F) Western blot was used to assess the protein level of Bcl-2, Bax, pro-Caspase-3 and cleaved-Caspase-3 in different gruops. (G) RT-qPCR was used to detect mRNA of Bcl-2, Bax, pro-Caspase-3 and cleaved-Caspase-3. * P<0.05, ** P<0.01, versus control group.
