Figure 3.

Knockdown of miRNA-21-5P in GC9811 cells. Depletion of endogenous miR-21-5p in GC9811 cells was performed by lentiviral transfection using specific small interfering RNA targeting miR-21-5p or RNAi-Vector. Total RNA was extracted at 48 h post-transfection and subjected to reverse transcription-quantitative polymerase chain reaction analysis with miR-21-5p-specific primers. Values are expressed as the mean ± standard deviation Each sample was analyzed in triplicate and normalized to 5S. ***P<0.001 vs. RNAi-Vector group. RNAi, RNA interference; miRNA/miR, microRNA; RNAi-Vector, scrambled control vector.