Figure 2.

Chronic cadmium exposure reduced neutrophil infiltration in the wounds of mice. Mice were given drinking water containing 0 or 30 mg/l CdCl₂ for 8 weeks. A, H & E staining of uninjured skin tissues. Wounded tissues at 3 h after skin punch were examined for neutrophil infiltration by H & E staining (B), myeloperoxidase (MPO) immunostaining (C,D), and MPO activity assay (E). All experiments were repeated at least 3 times with similar results and representative data are shown. MPO positive cells were analyzed in 3 random fields under microscope for each section using Image-Pro Plus. Five sections from 5 animals each group were analyzed. (A–C) show representative images of H & E staining and MPO immunostaining, respectively, scale bar = 50 µm. Data in (D) and (E) are mean ± SE, n = 5 per group. *P < .05, **P < .01, compared with untreated mice.