Table 1.
Fluorescence derivatizations used in HPLC and CE with DNS-chloride: 1-dimethyl-aminonaphthalene-5-sulfonyl chloride, FQ: 3-(2-furoyl)quinoline-2-carboxaldehyde, NBD-F: 4-fluoro-7-nitrobenzofurazan, OPA: ortho-phtalaldehyde and NDA: napthalene-2,3-dicarboxaldehyde. Cl : naphthalene.
| Analyte | Matrices | Derivatizing reagent | Separation Type | Temperature condition (°C) | Reaction time | λex-λem (nm) | Limit of detection | Refs. |
|---|---|---|---|---|---|---|---|---|
| Phospholipids with amino group | ||||||||
| GPEtn, lyso-GPEtn, GPSer, lyso-GPSer | Rat brain | DNS-chloride | HPLC | 50 | 3h | 342-500 | 20 pmol | [29] |
| GPEtn | Egg | DNS-chloride | HPLC | 50 | 2-3h | 360-> 420 | - | [30,31] |
| GPEtn, lyso- GPEtn, GPSer, lyso- GPSer | Rat brain | succinimidyl 2-naphtoxyacetate | HPLC | Room temperature | 2h | 228-342 | 2 pmol | [32] |
| GPEtn, lyso- GPEtn, PS, lysoPS | - | FQ | MECC | 55 | 15min | 488-630 | 0.65-1.1 fg | [12] |
| PE, lysoPE, PS, lysoPS | - | NBD-F | MECC | 55°C | 5min | 488-535 | 0.18-0.87 fg | [12] |
| Sphingoid base 1-phosphates | Human plasma, serum, platelets | OPA | HPLC | Room temperature | 20 min | 340-455 | < 5 pmol | [33] |
| Sphingosine 1-phosphate | Human plasma | OPA | HPLC | Room temperature | 20 min | 340-455 | < 50 ng/mL | [34] |
| Sphingosine 1-phosphate | Plasma (human, horse, mouse), mousse tissues | NDA | HPLC | 50°C | 10 min | 252-483 | 20.9 fmol | [35] |
| Phosphatidic acid | ||||||||
| GPA | Egg | DNS-ethanolamine from DNS-Cl | HPLC | Room temperature | 2-3h | 360-420 | - | [31] |
| GPA | Rabbit platelets | 3-(9 anthroyl) diazo-2-propene | HPLC | 4°C | 8h | 254-430 | 0.05ng/mL | [36] |