Table 3.
Fluorescence post-column derivatizations used in HPLC.
| Refs. | [51] | [53] | [61] | [62] | [63] | [64] | [54] |
|---|---|---|---|---|---|---|---|
| Molecular probe | BBOT | DPH | DPH | DPH | DPH | DPH | DPH |
| Concentration before | 0.023 | 0.15 | 0.45 | 0.00045 | 0.45 | 10.5 | 3.35 |
| (and after) mixing (µmol/L) | -0.006 | -0.13 | -0.34 | -0.00025 | -0.37 | -7 | -2.79 |
| λex-λem (nm) | 372–440 | 365–460 | 340–460 | 340–460 | 340–460 | 340–460 | 350–430 |
| Surfactant | - | - | Tween 20 | Tween 20 | Brij 35 | Brij 35 | Brij 35 |
| Concentration (v/v) | 0.00% | 0.00% | 0.03% | 0.03% | 0.02% | ||
| Flow (mL.min-1) | |||||||
| - Chromatographic | 0.8 | 1 | 1 | 1 | 1 | 1 | 0.1 |
| - Post-column phase | 0.25 | 6 | 3 | 1,2 | 4.5 | 2 | 0.5 |
| % aqueous phase | 1.5 | 87.9 | 75 | 54.5 | 82.4 | 66.7 | 83.3 |
| Tubing L(m) × id(mm) | 0.3 × 0.25 | - | 3 × 0.5 | 0.3 × 0.5 | 2 × 0.8 | 3 × 0.25 | 1.4 × 0.5 |
| Time (s) | <1 | 60 | 9 | 2 | 11 | 3 | 27 |
| Temperature (°C) | - | 40 | 50 | 50 | 50 | 50 | 35 |