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. 2018 Nov 20;5:99. doi: 10.3389/fmolb.2018.00099

Figure 8.

Figure 8

Altered TF associated protein complexes in 3xTg-AD mice cortex and hippocampus, and significantly reduced P-TF in CaMKK2 KO mice serum. (A) Immunoblots showing TF associated MPCs in the cortex and hippocampus tissues from 14 months old wild-type and 3xTg-AD mice. The Coomassie stained gel strip on the top panel showing native page molecular weight ladder. Colored squares represent a change in the relative intensity. (B) Scatter plot showing relative intensities of ~1,000 kDa TF associated MPC. The intensity of the ~720 kDa complex was used for normalization. (C) Immunoblot showing total TF level in the serum (top panel). Bottom panels: SDS-PAGE gel stained with Oriole to show total protein loading. (D) Immunoblots showing charged fractions of TF in the serum. Proteins were focused on IPG pH3-10NL strips (top panel) and IPG pH3-6L strips (bottom panel), respectively. The red dotted rectangle indicates P-TF (pH~3-4 fractions). Blue dotted rectangles indicate HMW TF. (E) Scatter plot showing relative abundance of P-TF in serum. The pH~3-4 fractions were normalized on the basis of pH~6 fraction intensities (green rectangle) in IPG pH3-6L strips. N = 6 replicates from 3 mice in all experiments. The p-value by t-test (unpaired).