Table 1.
List of Antibodies and other reagents.
| Name | Description | Source | Catalog number | ||
|---|---|---|---|---|---|
| CaMKK2 HDR plasmid (Human) | Homology arm with selection/reporter cassette for HDR | SCBT | Sc-400928-HDR | ||
| CaMKK2 CRISPR/Cas9 KO plasmid (Human) | Pool of 3 plasmids each encoding ccas9 nuclease and a target specific 20nucleotide gRNA | SCBT | Sc-400928 | ||
| Cre vector | Cre recombinase expressing plasmid | SCBT | Sc-418923 | ||
| mCherry-TFR | C-terminal mCherry tagged TFR | Addgene | 55144 | ||
| Flag-TF cDNA | Human Transferrin full length, N-terminal Flag tag | Gene Script | OHu26129 | ||
| CaMKK2 siRNAs: s135956, s135958, s135957 | Rat CaMKK2 specific siRNAs: CGAGCAGGTGTATCAGGAA, CAAAGGCATTGAGTACTTA, CCCGGATGTTGGACAAGA | ThermoFisher Scientific | 4390771 | ||
| Name | Source | Type | Host Sp. | Cat. No | Lot No. |
| Antibodies | |||||
| CaMKK2(ZZ9) | SCBT | Monoclonal | Mouse | Sc-100364 | I2914 |
| B-23(NA24) | SCBT | Monoclonal | Mouse | Sc53175 | F2212 |
| VDAC1(B-6) | SCBT | Monoclonal | Mouse | Sc-390996 | C0116 |
| α-tubulin (TU-02) | SCBT | Monoclonal | Mouse | Sc-8035 | C2017 |
| ERK1/2(C-9) | SCBT | Monoclonal | Mouse | Sc-514302 | G3115 |
| Histone-1(G-1) | SCBT | Monoclonal | Mouse | Sc-395530 | G1514 |
| GAPDH(0411) | SCBT | Monoclonal | Mouse | Sc-47724 | 1015 |
| Flag | Sigma | Monoclonal | Mouse | F3165 | SLBT6752 |
| Phospho-Serine | SCBT | Monoclonal | Mouse | SC-81514 | I1615 |