Table 2.

List of phosphopeptides identified by LC-MS/MS analysis. log(e) for peptides represent the expectation value for the spectrum-to-sequence assignment.

Log(e)	m+h (Da)	Start	Sequence (human serotransferrin)	End	Modifications	No of times observed in GPM
GPM ID: GPM10000002014 (Scrambled control)
−15	4348.937	381	$\text{pS}$VGKIE$\text{cC}$VSAETTED$\text{cC}$IAKI$\text{oM}$NGEADA$\text{oM}$SL DGGFVYIAGK	420	$\text{S}381+\text{Phospho}$, C387+Carbamidomethyl, C396+Carbamidomethyl, M401+Oxidation, M408+Oxidation	2
−3.2	4412.908	381	$\text{pS}$VGKIECV$\text{pS}$AEpTpTED$\text{cC}$IAKIMNGEADA$\text{oM}$SLDGGFVYIAGK	420	$\text{S}\mathrm{381/389}\&\text{ T}\mathrm{392/393}+\text{Phospho}$, C387+C396 Carbamidomethyl, M408+Oxidation	2
−3.9	3364.386	391	$\text{dE}$pTpTED$\text{cC}$IAKI$\text{oM}$NGEADAM$\text{pS}$L DGGFVYIAGK	420	$\text{T}\mathrm{392/393}+\text{Phospho}$, $\text{S}409+\text{Phospho}$, E391+Dehydrated, C396+Carbamidomethyl, M401+Oxidation	815
−1.4	2337.942	490	INH$\text{cC}$RFDEFF$\text{pS}$EG$\text{cC}$APGSK	508	C493+Carbamidomethyl, $\text{S}500+\text{Phospho}$, C503+Carbamidomethyl	9,565
GPM ID: GPM10000002011 (Scrambled control)
−2.7	2785.36	251	RKPVDEpYKD$\text{cC}$HLAQVPSHTVVAR	273	$\text{Y}257+\text{Phospho}$, C260+Carbamidomethyl	105
−4.1	3689.642	333	pYLGpYEpYVTAIRNLREGT$\text{cC}$PEAPTDE$\text{cC}$KPVK	362	$\text{Y}\mathrm{333/336/338}+\text{Phospho}$, C350+Carbamidomethyl, C358+Carbamidomethyl	0
−5.8	3364.386	391	$\text{dE}$pTpTED$\text{cC}$IAKI$\text{oM}$NGEADA$\text{oM}$SLDGGFVYIAGK	420	E391+Dehydrated, $\text{T}\mathrm{392/393}+\text{Phospho}$, C396+Carbamidomethyl, M401/408+Oxidation	815
−2.4	2337.942	490	INH$\text{cC}$RFDEFF$\text{pS}$EG$\text{cC}$APGSK	508	C493+Carbamidomethyl, $\text{S}500+\text{Phospho}$, C503+Carbamidomethyl	9,565
−5.2	2621.123	509	KD$\text{pSpS}$L$\text{cC}$KL$\text{cC}$$\text{oM}$GSGLNL$\text{cC}$EPNNK	530	$\text{S}\mathrm{511/512}+\text{Phospho}$, C514+Carbamidomethyl, C517+Carbamidomethyl, M518+Oxidation, C525+Carbamidomethyl	743
−1.4	2605.128	509	KD$\text{pS}$SL$\text{cC}$KL$\text{cC}$MGSGLNL$\text{cC}$EPNNK	530	$\text{S}511+\text{Phospho}$, C514+Carbamidomethyl, C517+Carbamidomethyl, C525+Carbamidomethyl	743
−2	2258.052	572	NLNEKDYELL$\text{cC}$LDGpTRKP	589	C582+Carbamidomethyl, $\text{T}586+\text{Phospho}$	3

o, oxidation; c, carbamidomethyl; p, Phosphorylation; d, dehydration. The GPM ID numbers can be used to access MS-MS spectrum data at the Global Proteome Machine (GPM) database using the link: http://hs2.proteome.ca/tandem/thegpm_tandem.html.