Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Nov 27;5:180266. doi: 10.1038/sdata.2018.266

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018, The Author(s)

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ The Creative Commons Public Domain Dedication waiver http://creativecommons.org/publicdomain/zero/1.0/ applies to the metadata files made available in this article.

PMC Copyright notice

This heat map-like illustration shows the distribution of read counts within 500 bp intervals. This dataset shows that the Sequel sequencer resulted in longer average read lengths (~79% of the reads are longer than 1,500 bp). The size selection of the RSII samples had a significant effect on the average read lengths: even if most of the reads are within 1,000–2,000 bp, the distribution of the read counts is different: 0.8–2 kb: 87,560%; 2–3 kb: 73,493%; 3–5 kb: 73,543%; >5 kb: 69,487% of the reads are within this range. The MinION sequencing on the poly(A)-selected samples shows about the same read length distribution as the non-size-selected RSII (the majority of the reads is within the 1,000–2,000 bp interval, however, the distribution of the MinION reads is smoother. The Cap-selected samples and the direct RNA samples have a peak at the lower range: about 30–35% of the reads are between 501–1,000 and 1,001–1,500 bp in both samples. The random primed samples (RSII and MinION) generated the shortest average read lengths.