Skip to main content
. 2018 Nov 27;5:180266. doi: 10.1038/sdata.2018.266

Table 7. The list of reverse transcription primers used in this study.

Sequencing method Name, availability Catalog # Sequence (5′ -> 3′)
PacBio amplified PolyA 3' SMART CDS primer II A - SMARTer PCR cDNA Synthesis Kit (Clontech) 634925 & 634926 AAGCAGTGGTATCAACGCAGAGTAC(T)30VN
PacBio amplified Random Custome made (IDT DNA) AAGCAGTGGTATCAACGCAGAGTACNNNNNN (G: 37%; C: 37%; A: 13%; T: 13%)
MinION CAP selected TeloPrime Full-Length cDNA Amplification Kit (Lexogen) 013.08 & 013.24 TCTCAGGCGTTTTTTTTTTTTTTTTTT
MinION cDNA Poly(T)-containing anchored primer [(VN)T20 - ONT recommended, custom made (Bio Basic) 5phos/ACTTGCCTGTCGCTCTATCTTC(T)20VN
MinION Random Custome made (IDT DNA) 5Phos/ACTTGCCTGTCGCTCTATCTTCNNNNNN
MinION RNA RT adapter - Direct RNA Sequencing Kit (Oxford Nanopore Technologies) SQK-RNA001 GAGGCGAGCGGTCAATTTTCCTAAGAGCAAGAAGAAGCCTTTTTTTTTT