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. 2018 Nov 20;9:2687. doi: 10.3389/fimmu.2018.02687

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Copyright © 2018 Chiorean, Baican, Mustafa, Lischka, Leucuta, Feldrihan, Hertl and Sitaru.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Algorithm of measurement of fluorescence intensity in direct IF microscopy images, in patients with BP (n = 27 patients). The fluorescence intensity was assessed by two independent blinded observers using Image J software, 1.47v version. The fluorescence intensity at the basement membrane was analyzed by selecting 50 points (using Multi-point tool), each point being situated at the half distance between two previous points. Background fluorescence was evaluated by selecting the whole dermis area, using “Freehand selections” tool. In order to obtain the mean value and standard deviation of the fluorescence intensity for the 50 points on the basement membrane and dermis background we have used the Analyze-Measure-Summarize tool.