Table 1.
Gene | Forward primer (5′–3′) | Reverse primer (5′–3′) |
---|---|---|
| ||
GAPDH | GGTGAAGGTCGGAGTCAACGG | GGTCATGAGTCCTTCCACGAT |
Wnt5a | TCTCAGCCCAAGCAACAAGG | GCCAGCATCACATCACAACAC |
β-Catenin | GCTACTGTTGGATTGATTCGAAATC | CCCTGCTCACGCAAAGGT |
Axin2 | CCCCAAAGCAGCGGTGC | GCGTGGACACCTGCCAG |
Abbreviation: qRT-PCR, quantitative real-time transcription polymerase chain reaction.