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Images of SAGM-preserved RBCs stored in the presence of Trolox (125, 625 and 3,125 µmol/l) and in the absence of the antioxidant (control). Images were taken at day 1 and day 42. Magnification of 630× (inverted microscope (Axio Observer; Zeiss, Jena, Germany) equipped with digital camera and software for digital image processing) The percentages of shape-changed cells were evaluated by counting approximately 350 RBCs in randomly chosen fields.
