Figure 6.

The investigation between miR-455-5p and Rab31 mRNA was assessed using a dual luciferase reporter assay. Plasmids (0.5 µg) with WT or mutant 3′-untranslated region DNA sequences were co-transfected into Eca109 cells with miR-455-5p mimics. After cultivation for 24 h, ells were lysed using a dual luciferase reporter assay kit and the fluorescence intensity was measured using a GloMax 20/20 luminometer. Renilla was used as internal reference and the fluorescence values of each group of cells were measured. *P<0.05 vs. NC group. miR, microRNA; ESCC, esophageal squamous cell carcinoma; NC, negative control.