Figure 2.

Overexpression of GATA4 promotes nasopharyngeal cancer cell migration and invasion. 5-8F cells were transfected with an empty vector, FLAG-GATA4, SCR or siGATA4. (A) The protein expression of GATA4 was detected using western blotting. (B) The relative mRNA expression levels of GATA4 were detected and quantified using reverse transcription-quantitative polymerase chain reaction. (C) Transwell invasion and (D) wound healing assays were performed to determine the effect of GATA4 expression on cell invasion and migration, respectively (magnification, ×20). *P<0.05. FLAG-GATA4, vector overexpressing GATA4; si, small interfering RNA; SCR, scramble siRNA; siGATA4, siRNA against GATA4.