Figure 2.

Heat stroke impairs the immunosuppressive capacity of splenic Tregs. (A) Splenocytes were harvested at 24 h post-heat stress, and splenic Tregs and CD4⁺T cells were sorted via FACS analysis. The proliferation rate of CD4⁺T cells was defined by BrdU⁺CD4⁺T cells. (B) The proliferation rate of CD4⁺T cells was increased in the HS group compared with NC group at different ratios. Data are expressed as the mean ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001. NS, not significant; NC, negative control; HS, heat stroke; CD, cluster of differentiation; Tregs, T regulatory cells; BrdU, bromodeoxyuridine; PE, phycoerythrin.