Figure 3.

Heat stroke downregulates the expression levels of CTLA4 and CD39/CD73 in splenic Tregs. (A and B) Splenic CTLA4⁺, (C and D) CD39⁺ and (E and F) CD73⁺Tregs were defined as CD4⁺Foxp3⁺ CTLA4⁺, CD4⁺Foxp3⁺ CD39⁺ and CD4⁺Foxp3⁺ CD73⁺splenocytes in FACS. Percentages of CTLA4⁺, CD39⁺ and CD73⁺Tregs were decreased in the HS group at 0, 24 and 72 h time intervals post-heat stress compared with the NC group. Data are expressed as the mean ± standard deviation. *P<0.05, **P<0.01 and ***P<0.001 vs. NC. NC, negative control; HS, heat stroke; Foxp3, forkhead/winged-helix transcription factor; CD, cluster of differentiation; Tregs, T regulatory cells; CTLA4, cytotoxic T-lymphocyte associated protein 4; APC, allophycocyanin; PE, phycoerythrin.