Fig. 5.

—Factors influencing ldcA expression. Activity of ldcA promoter fused to lacZ reporter gene was assessed during growth in different media and genetic backgrounds. Measurements of the β-galactosidase activity of PAO1::PldcA-lacZ strain grown either in minimal medium P (MMP) containing 20 mM l-glutamate and 20 mM arginine (A), or in LB containing or not 20 mM arginine (B) were performed at times indicated. β-Galactosidase activity is expressed in Miller Units (left Y-axis) and presented in the bar graphs. Growth was performed in 125 ml flasks, followed by measure of OD₆₀₀ (right Y-axis) and plotted on lines. Results are the average of values from three independent experiments ± standard deviation (SD).