Fig. 6.

—Intracellular Cad in rich medium is produced by the lysine decarboxylase LdcA. (A) Growth curves of the wild-type strain, the ldcA mutant and the complemented strain in the rich Mueller Hinton medium. Data show a representative experiment from four independent biological replicates. (B) Intracellular concentrations, expressed in area of chromatogram peak, of the three indicated polyamines at 1) early-, 2) mid-, and 3) late-exponential growth phases as indicated, measured in duplicate in four biological replicates.