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. 2010 Jul 7;15(7):4757–4772. doi: 10.3390/molecules15074757

Figure 3.

Figure 3

Inhibition of 5’UTR dimerization in vitro by chimeric inhibitor RNAs derived from both hammerhead and hairpin ribozymes [A) and B), respectively). The 5’UTR dimerization was performed in the presence and absence of inhibitor RNAs. Dimerization reactions were carried out at 37 ºC for 20 min with 157 nmol of internally radiolabelled 5’UTR RNA and a 20-fold excess of inhibitory RNA. The reactions were resolved in 4% polyacrylamide native gels. Line 1 in both gels represents non-dimer condition controls. Line 2 represents 5’ UTR dimerization in the absence of any RNA inhibitor. The shifted band within line 2 with respect to line 1 is considered as the dimer.