Figure 4.

Ex vivo inhibition of HIV-1 viral production. The HEK293T cells were transfected with the pNL4.3 plasmid and an inhibitory RNA expressing vector at a molar ratio of 1:10 A) or with an in vitro synthesized RNA B). The bar graph represents the percentage of viral protein p24 levels in the supernatant 48 hours after transfection relative to the control. The inhibitory RNA is indicated below each bar. The empty pU6 vector and an RNA molecule consisting of both U6 snRNA flanking hairpins without any sequence in between were used as negative controls in A) and B), respectively. The results are the means of three independent experiments (** p < 0.01).