Figure 3.

Polymers enhance quorum sensing in V. cholerae. V. cholerae A1552 containing pBB1 was grown for 16 h and then adjusted to an OD600 of 0.2 in the presence of P1 (A–C), P2 (D–F), or AMW alone (G). Polymers were adjusted to final concentrations of 0.005 (A, D), 0.05 (B, E), or 0.5 (C, F) mg mL^–1 in AMW. Luminescence of samples was imaged every 30 min for 15 h, and representative images for each time point are shown from 0 h (top left) to 15 h (bottom right) of each panel. Luminescence intensities over time were analyzed by quantifying pixel intensities, and means ± SEM from at least three biological replicates are shown for P1 (left) and P2 (right panel) (H).