Table 2. Plasmids Used in This Study.
plasmid | description | source or ref |
---|---|---|
pRW50T | pRW50 derivative with a oriT sequence from pRK2; TetR | gift from D. Grainger |
pRW50T-rbmA | pRW50T containing 273 bp of the upstream region of rbmA, cloned between EcoRI and HindIII restriction sites; TetR | this study |
pRW50T-vpsR | pRW50T containing 195 bp of the upstream region of vpsR, cloned between EcoRI and HindIII restriction sites; TetR | this study |
pRW50T-aphA | pRW50T containing the upstream region of aphA, cloned between EcoRI and HindIII sites; TetR | (12) |
pBB1 | luxCDABE cosmid; TetR | (35) |