Figure 5.

Analytical HPLC and mass spectrometric analysis of the purified entry inhibitor Myr-HBVpreS/2-78. The HPLC chromatogram shows one single peak corresponding to the purified peptide. Insert: mass spectrum obtained (22.37–23.05 min). The signals obtained show the 4-fold charged m/z signals belonging to the desired peptide. The exact monoisotopic mass was calculated to be [M+4H]⁴⁺ = 2166.3504 (measured: [M+4H]⁴⁺ = 2166.3487). Besides the product, one side product was identified to be a peptide missing an aspartic acid in the peptide sequence ([M+4H]⁴⁺ = 2137.5931). Conditions: Hypersil Gold C₁₈ column; linear gradient of 0.1% TFA in water to 0.1% TFA in acetonitrile in 30 minutes, flow rate of 0.2 mL/min, 60 °C, wavelength of 214 nm.