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. 2018 Oct 11;16(6):5123–5129. doi: 10.3892/etm.2018.6851

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Copyright: © Zhai et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — Determination of Ag85A mRNA expression levels by RT-qPCR. Ag85A expression in DC 2.4 cells transfected with pc-DNA3.1, DC2.4 plus pcDNA3.1 (mock-DC) or non-transfected DC 2.4 (Con) for 24 h was determined by RT-qPCR. Data are presented as the mean ± standard error of the mean from three independent tests. **P<0.01 vs. mock-DC group. DC, dendritic cell; Ag85A, antigen 85A; Con, control; RT-qPCR, reverse transcription-quantitative polymerase chain reaction.