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. 2018 Sep 18;16(6):4655–4663. doi: 10.3892/etm.2018.6752

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Copyright: © Yan et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — TGFBI is a direct miR-21-5p target gene. (A and B) Western blot analysis of TGFBI protein levels in A549 cells and H1299 cells after transfection with the control mimic, miR-21-5p mimic, control inhibitor, or miR-21-5p inhibitor. (A) Representative image. (B) Statistical analysis of the image. (C) RT-qPCR analysis of miR-21-5p level in A549 cells and H1299 cells transfected with the control mimic, miR-21-5p mimic, control inhibitor, or miR-21-5p inhibitor. (D) RT-qPCR analysis of TGFBI mRNA level in A549 cells and H1299 cells transfected with the control mimic, miR-21-5p mimic, control inhibitor, or miR-21-5p inhibitor. The results were expressed as mean ± SD of three independent experiments (n=3;*P<0.05; **P<0.01; ***P<0.001).