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Patterned 3-D co-culture of hepatocyte spheroids and endothelial cells (BAECs). (a) Micro-patterned α-lactosyl-PEG/PLA coated dish with ϕ100 μm circular domains spaced in l100 μm intervals. (b) Patterned culture of BAECs on substrate (a) for 24 h at 37 °C. (c) Organized pattern of hepatocytes spheroids underlaid with BAECs. (d) Hepatocytes directly seeded on substrate (a) without pre-adhered BAECs [28].
