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Viability assay by LIVE/DEAD Viability/Cytotoxicity assay kit of three dimensionally co-cultured spheroids on α-lactosyl-PEG/PLA-pattern-coated dishes for three weeks at 37 °C. (a) Live cell image stained with calcein. (b) Dead cell image stained with EthD-1. (c) Distinct nuclei stained with a DNA-binding dye (Hoechst 33342) [28].
