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. 2010 Sep 16;15(9):6436–6451. doi: 10.3390/molecules15096436

Figure 2.

Figure 2

Effects of chrysophanol on the level of IL-6 and COX-2 in DSS-treated colon tissue. Experimental colitis in mice was induced by a 5% DSS dissolved in the drinking water for 7 days. Chrysophanol was administered at doses of 5 mg/kg once a day for 7 days prior to 5% DSS supplement. At the end of experiment, the colon tissues were cut out and homogenized. (A) The levels of IL-6 in the indicated groups were measured by ELISA; (B) The levels of COX-2 were evaluated by Western blot analysis; (C) The relative expression level of COX-2 was measured using an image analyzer.1) Control group; 2) DSS alone-treated group; 3) chrysophanol (5 mg/kg) + DSS treated group; 4) sulfasalazine(150 mg/kg) + DSS treated group. Values were represented in the mean ± S.E.M. (n = 5) of duplicate determinations from triplicate separate experiments (#p < 0.05 vs. control, *p < 0.05 vs. DSS alone).